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MIQE Guidelines: A roadmap for proper qPCR experimental design and reporting
Quantitative real-time PCR (qPCR) is today's most widely used molecular technology, and continuous improvements to chemistries, enzymes, master mixes, and instruments have led to an increasingly reliable assay that virtually guarantees results. However, the same improvements that make it easy to generate qPCR data often conceal experimental practices that have resulted in the regular publication of data that are inconsistent, inaccurate and, sometimes, simply wrong. Incomplete reporting of experimental detail further confounds assessment of qPCR data validity, calling into question scientific conclusions that serve as a basis for further basic research and diagnostic applications.
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