As leaders in their respective fields, IDT and
This partnership offers you:
DNA origami self-assembly requires 2 types of DNA, a scaffold strand and a mixture of staple strands. A scaffold strand is a long piece of single-stranded DNA. Staple strands are single-stranded 30–80
The single-stranded Scaffold DNAs are produced by
Because DNA origami design is complex, we recommend that new users contact the
In addition to offering the above 6 predesigned structures,
Staple strand oligos are synthesized using IDT specialized, proprietary oligo synthesis platforms and are each verified by ESI-mass spectrometry.
IDT's long-standing reputation as a pioneer and leader in custom oligo manufacturing is largely due to our vertically integrated, proprietary synthesis platforms. Nearly every component of our manufacturing process is designed and developed in-house, including specialized synthesizers that can accommodate the most demanding oligo requests and high-throughput automation systems that ensure the fastest turnaround times possible. We do not rely on third-party manufacturers for any of the machines or chemical reagents used for synthesis, allowing us to easily monitor and refine instruments and reagents with ease. Our ability to control these variables allows us to produce oligos that are unmatched in quality and consistency.
An important measure of quality for any oligo manufacturer is coupling
Figure 1. Higher coupling efficiency leads to more full-length product. The relationship between increasing coupling efficiency and increasing full-length product is shown. DNA oligos from IDT are synthesized on proprietary platforms that deliver a coupling efficiency (99.4%) that exceeds industry standards (98.50%) and thus results in a greater proportion of full-length product.
Figure 2. Consistent, high coupling efficiency maintained at all IDT manufacturing sites. Many variables can lead to reduced oligo quality, including ambient temperature, humidity, and input reagents. We track coupling efficiency at all of our manufacturing sites to ensure consistent quality with change in variables. A 30-day rolling average is shown.
For additional information about IDT DNA oligo production and quality, see Custom DNA Oligos.
Because DNA origami design is complex and benefits from specialized expertise, we recommend that new users contact
Web-based software tools are available for investigators interested in performing their own DNA origami design and analysis. DNA origami experts at
Please note: IDT Scientific Applications Support does not currently support questions regarding DNA origami design, construction, use, or performance. If you have questions, please refer to the FAQs section of this page (below, under Resources), or contact