Start with solid bases
Use oPools Oligo Pools for accurate, reliable, and affordable CRISPR libraries; primer pools for multiplex PCR; gene construction; data storage; and FISH analysis. These pools of custom single-stranded DNA sequences offer high fidelity, uniformity, low error rates, and low dropout rates. This means that you can avoid amplification bias, varying concentrations, or high error rates that are often encountered when using pooled oligos from other suppliers.
Available in convenient sizes:
|Scale (pmol/oligo)||Number of oligos per pool||Oligo length (bases)|
|1||100 to 20,000||40 to 350|
|10||10 to 2000||40 to 350|
|50||2 to 384||40 to 350|
* Regardless of pool size, the first 3300 bases will be charged at the oPools DNA Base 1 rate. Bases 3301–50,000 will be charged at the oPools DNA Base 2 rate, bases 50,001–100,000 will be charged at the oPools DNA Base 3, and bases more than 100,000 will be charged at the oPools Base 4 rate.
oPools Oligo Pools are pooled, high-fidelity single-stranded DNA sequences that are compatible with a variety of applications.
Proprietary DNA synthesis equipment permits rapid, high quality synthesis of nucleic acids. This platform is the same proprietary synthesis platform used to make IDT Ultramers. It uses an "extra rich" synthesis cycle for long oligos. Along with this refined synthesis cycle, oPools DNA oligos use a unique solid support specifically optimized to synthesize high-quality oligos up to 350 bases in length. Overall, these improvements to our already established manufacturing methods allow you to acquire longer, purer oligos for your research.
|Scale (number of oligos)|
|Mixed bases ||N = A, C, G, and T|
K = G and T
• Limited to 9 mixed bases per oligo
|QC and quantification||None|
|Shipping container||2 mL tube|
Figure 1 illustrates how our high coupling efficiency results in the exceptional quality of oPools oligo pools.
Figure 2 illustrates the yield uniformity of oPools oligo pools.
The high sequence representation is explained by very low dropout rates during synthesis. This is illustrated in Figure 3.