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Using synthetic, double-stranded DNA fragments will save you time and money compared to traditional cloning methods. We offer a variety of gene fragments, ranging from eBlocks™ Gene Fragments for high-throughput antibody research to gBlocks™ HiFi Gene Fragments for large gene construction. We have quick and reliable solutions to meet your experimental needs.
Use Table 1 to help choose the best double-stranded DNA product for your experiment.
Table 1. Comparison of IDT gene fragments.
|eBlocks Gene Fragments||gBlocks Gene Fragments||gBlocks HiFi Gene Fragments|
|Length||300–900 bp||125–3000 bp||1000–3000 bp|
|Median Error Rate||1:5000||1:5000||1:12,000|
Turnaround time (BD)*
|Yield||200 ng||250–1000 ng||1000 ng|
|Recommended applications||Antibody discovery|
High throughput screening
|Large gene construction,|
including pathway development and microbe design
* BD = business days; N/A = not applicable (eBlocks fragments are not available in tubes)
† NGS = next generation sequencing
Using IDT gene fragments will reduce the time and expense of screening colonies compared to fragments from other suppliers (Table 2). Cloning efficiency is affected by many factors, including the cloning method used, the stability of the cell line and plasmid, vector preparation, and toxicity or stress from expression of coding sequences. The values in Table 2 represent typical screening numbers needed when using a seamless assembly method, such as Gibson Assembly® (Synthetic Genomics) or NEBuilder® HiFi assembly (New England BioLabs), and when issues mentioned above are not significant contributors to error or selection.
Table 2. Approximate number of colonies to screen for a 90% chance of getting a correct clone.
gBlocks fragments are the original and preferred choice for gene assembly, genome editing, qPCR standards, and more. For larger gene construction projects, use high-fidelity gBlocks HiFi fragments.