Processing
xGen UDI-UMI Adapters
Single-use, TA-ligation-ready, full-length adapters
xGen UDI-UMI Adapters combine the accuracy of unique dual indexing with the convenience of single-use format for a variety of sequencing applications on Illumina instruments. These full-length adapters contain 8-base unique dual indexes (UDI) that minimize read misassignment. An optional 9-base unique molecular identifier (UMI) can be used for quantitative assays or low-frequency variant detection. The adapters are available both unmethylated and methylated for bisulfite sequencing. Full-length adapters support PCR-free and PCR-amplified library preparation methods.
Our NGS quality control minimizes risk of misassignment
UDI is the current standard for data accuracy and confidence
UMIs are included for sensitive applications
Adapters are pre-configured and ready to ship
Product details
xGen UDI-UMI Adapters include P5 and P7 sequences that are compatible with Illumina sequencing platforms. These full-length NGS adapters are compatible with the Lotus DNA Library Prep Kit and other TA-ligation-based library preparation methods (Figure 1). The adapters contain a 9-base degenerate unique molecular identifier (UMI) in an optional read position. The UMI sequence can be used for quantification (counting applications like RNA-seq and ChIP-Seq) and error correction (low-frequency variant detection). The UMI is downstream of the i7 sample index, making the UMI data/information optional. This unique design provides the ability to run the adapter in 3 different schemes: single index, unique dual index, and unique molecular identifier modes.
Figure 1. xGen UDI-UMI Adapters.
Specifications
| Sample index length | 8-base non-redundant sample indexes |
| Number of indexes | 16 or 96 unique dual indexes (UDIs) per kit |
| Concentration | 15 µM |
| Shipping container | 96-well plates |
| Reactions per well | 1 (to prevent cross-contamination between indexes) |
| Adapter modifications | Methylated options available for bisulfite sequencing* |
| QC | NGS |
| Illumina platform compatibility | 2- and 4-color platforms |
* For bisulfite sequencing, see our FAQ for additional information.
Shipping and storage
Ships on dry ice
Turnaround time: typically ships within 1 business day
Storage temperature: –20°C
Performance
Figure 2. Uniform genome coverage for PCR-free and PCR-amplified libraries. Libraries were prepared using 100 ng of human gDNA (NA12878, Coriell) and full-length xGen UDI-UMI Adapters (IDT) with or without PCR amplification (4 cycles) and sequenced on the MiSeq® (PCR-free) and HiSeq® 4000 (amplified) platforms (Illumina). Normalized coverage of each library is shown as dark and light blue lines, the expected normalized coverage of 1.0 is indicated with a dotted line,
and the number of 100 bp regions at each GC% is shown as a histogram in grey.
Resources
User guides and protocols
Index files
Data analysis guidelines
Brochures and flyers
Frequently asked questions
Webinars
Posters
Certificates of analysis (COAs)
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