Initiation of isothermal helicase-dependent amplification of a rpoB gene sequence by RNase H2-mediated cleavage of blocked DNA primers.
IDT MiniGene constructs were used as microRNA (miR) sponges against miR-17, miR-18a, miR-19, and miR-92. The sequences were also designed with 5′ Xho1 and 3′ EcoR1 sites for subcloning into other vectors by restriction digest.
Use of DsiRNAs to inhibit viral replication of metapneumovirus in vitro and in vivo. The authors report highly specific inhibition achieved without induction of cytokines or off-target effects.
Ultramer Oligonucleotides were used for BAC mutagenesis via a two-step l–red mediated recombination strategy.
IDT MiniGene synthetic genes were engineered for use in transcription factor binding assays. Mutant sites were created for Sp1, HNF-1, and NF-Y and the MiniGene synthetic genes were provided in pIDTSMART-KAN vectors.
Use of an RNA aptamer–siRNA chimera that triggers sequence-specific degradation of HIV RNAs to suppress HIV-1 replication and prevent CD4+ T cell depletion in humanized mice.