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Citations of IDT products

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322 Citations found

Zhang X, Song Y, Shah AY, Lekova V, Raj A, Huang L, Behlke MA, and Tsourkas A. (2013) Quantitative assessment of ratiometric bimolecular beacons as a tool for imaging single engineered RNA transcripts and measuring gene expression in living cells. Nucleic Acids Res, 41 : e152.
Keiser MS, Geoghegan JC, Boudreau RL, Lennox KA, Davidson BL. (2013) RNAi or overexpression: alternative therapies for spinocerebellar ataxia type 1. Nuerobiol Dis, 56 : 6–13.
Dahan L, Huang L, Kedmi R, Behlke MA, and Peer D. (2013) SNP detection in mRNA in living cells using allele specific FRET probes. PLoS ONE, 8 : e72389.
Tetreault P, Beaudet N, Perron A, Belleville K, Rene A, Cavelier F, Martinez J, Stroh T, Jacobi AM, Rose SD, Behlke MA, and Sarret P. (2013) Spinal NTS2 receptor activation reverses signs of neuropathic pain. FASEB J, 27 : 3741-3752.
Fernandez G, Zaikos TD, Khan SZ, Jacobi AM, Behlke MA, Zeichner SL. (2013) Targeting Iκβ proteins for HIV latency activation: the role of individual Iκβ and NF-κβ proteins. J Virol, 87 : 3966–3978.
Singh R, Low ET, et al. (2013) The oil palm SHELL gene controls oil yield and encodes a homologue of SEEDSTICK. Nature, 500 : 340–344.

gBlocks Gene Fragments are used to generate constructs for yeast two-hybrid assays.

Goraczniak R, Wall BA, Behlke MA, Lennox KA, Ho ES, Zaphiros NH, Jakubowski C, Patel NR, Zhao S, Magaway C, Subbie SA, Jenny Yu L, Lacava S, Reuhl KR, Chen S, Gunderson SI.. (2013) U1 adaptor oligonucleotides targeting BCL2 and GRM1 suppress growth of human melanoma xenografts in vivo. Mol Ther Nucleic Acids, 14 : e92.
Carlson CS, Emerson RO, et al. (2013) Using synthetic templates to design an unbiased multiplex PCR assay. Nat Commun, 4 : 2680.

The authors have developed a multiplex PCR method that allows for quantitative analysis of T- and B- cell receptor diversity using next generation sequencing. gBlocks Gene Fragments are used as templates for optimization of the multiplex primer mix, a critical step for maintaining quantifiable differences and detecting low level transcripts. 

Ramachandran S, Karp PH, Jiang P, Ostedgaard LS, Walz AE, Fisher JT, Keshavjee S, Lennox KA, Jacobi AM, Rose SD, Behlke MA, Welsh MJ, Xing Y, McCray PB Jr. (2012) A microRNA network regulates expression and biosynthesis of wild-type and DeltaF508 mutant cystic fibrosis transmembrane conductance regulator. Proc Natl Acad Sci USA, 109 : 13362–13367.
Gantier MP, Stunden HJ, McCoy CE, Behlke MA, et al. (2012) A miR-19 regulon that controls NF-kB signaling. Nucleic Acids Res, 40 : 8048–8058.
Janas MM, Wang B, Harris AS, Aguiar M, Shaffer JM, Subrahmanyam YV, Behlke MA, Wucherpfennig KW, Gygi SP, Gagnon E, Novina CD. (2012) Alternative RISC assembly: binding and repression of microRNA-mRNA duplexes by human Ago proteins. RNA, 18 : 2041–2055.
Hicke B, Pasko C, et al. (2012) Automated detection of toxigenic Clostridium difficile in clinical samples: isothermal tcdB amplification coupled to array-based detection. J Clin Microbiol, 50 (8) : 2681–2687.

Use of RNase H2–dependent PCR to amplify and detect the tcdB gene in a region of the pathogenicity locus of C. difficile, with unknown sequence variation and insertions/deletions.

Zhou J, Song M-S, Jacobi AM, Behlke MA, Wu X, Rossi JJ. (2012) Deep sequencing analyses of DsiRNAs reveal the influence of 3’ terminal overhangs on dicing polarity, strand selectivity and RNA editing of siRNAs. Mol Therapy Nucleic Acids, 1 : e17.
Hernandez FJ, Stockdale KR, Huang L, Horswill AR, Behlke MA, McNamara JO. (2012) Degradation of nuclease-stabilized RNA oligonucleotides in mycoplasma-contaminated cell culture media. Nucleic Acid Ther, 22 : 58–68.
Cartwright CP, Lembke BD, et al.. (2012) Development and validation of a semiquantitative, multitarget PCR Assay for diagnosis of bacterial vaginosis . J Clin Microbiol, 50 : 2321–2329.

Ultramer Oligonucleotides were used to construct calibration standards. Each Ultramer contained a target sequence from one of the transcripts under study. All qPCR assays included a set of 3 Ultramer calibrators.

Pichu S, Krishnamoorthy S, et al. (2012) Dicer-substrate siRNA inhibits tumor necrosis factor alpha secretion in Kupffer cells in vitro: In vivo targeting of Kupffer cells by siRNA-liposomes . Pharmacol Res, 65 : 48–55.

Use of DsiRNAs to inhibit TNF-ɑ secretion in the study of inflammatory diseases. Data shows greater effectiveness of 27mer DsiRNAs than 21mers.

Pichu S, Krishnamoorthy S, et al. (2012) Knockdown of Ki-67 by Dicer-substrate small interfering RNA sensitizes bladder cancer cells to curcumin-induced tumor inhibition . PLoS One, 7 : e48567.

Bladder cancer cells pre-treated with Dicer-substrate siRNA (DsiRNA) against Ki-67, a DNA-binding protein associated with cell proliferation proliferation, promotes cell cycle arrest and enhances curcumin-induced apoptosis.

Krishnamurthy S, Behlke MA, Ramachandran S, Salem AK,McCray PB Jr, Davidson BL. (2012) Manipulation of cell physiology enables gene silencing in well-differentiated airway epithelia. Mol Ther Nucl Acids, 1 : e41.
Munoz-Alarcon A, Guterstam P, Romero C, Behlke MA, Lennox KA, Wengel J, El Andaloussi S, Langel U. (2012) Modulating anti-microRNA-21 activity and specificity using oligonucleotide derivatives and length optimization. ISRN Pharm, 2012 : e407154.
te Boekhorst BCM, Jensen LB, et al. (2012) MRI-assessed therapeutic effects of locally administered PLGA nanoparticles loaded with anti-inflammatory siRNA in a murine arthritis model . J Control Release, 161 : 772–780.
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