The xGen Human ID Research Panel enables unique identification of individual samples from a larger population. The panel provides genomic target enrichment of a distinctive set of single nucleotide polymorphisms (SNPs), including high minor allele frequency SNPs and a set of 5 gender marker SNPs. With a power of discrimination greater than 1 in 5 million, the Human ID Research Panel is compatible with various sample types, including cell-free DNA and DNA from formalin-fixed, paraffin-embedded (FFPE) tissues.
Capturing 76 distinct, highly polymorphic sites, the xGen Human ID Research Panel enriches a definitive set of single nucleotide polymorphisms (SNPs) selected to identify a unique individual from a population of over 5 million biological samples. The panel has been tested with DNA from formalin-fixed, paraffin-embedded (FFPE) tissues, cell-free DNA (cfDNA), and other sample types.
The xGen Human ID Research Panel comprises 229 individually synthesized xGen Lockdown Probes, each quality controlled by ESI mass spectrometry. The panel is also functionally validated to ensure consistent and reliable results. The xGen Human ID Research Panel can be combined with any xGen Lockdown Panel or custom xGen Lockdown Probe set as a spike-in panel. Panels of various sizes have demonstrated no compromise in on-target performance and coverage uniformity with the addition of this panel. The Human ID Research Panel can also be run as a standalone* panel to confirm sample identity before performing larger, more expensive sequencing studies.
Use the xGen Human ID Research Panel to:
xGen Lockdown Panels and Probes for diagnostic purposes are also available. Contact GMPinfo@idtdna.com for more information.
* The xGen Human ID Research Panel contains a relatively small number of probes. When used as a standalone panel, expect lower on-target capture results than when used as a spike in to a larger panel. This reduced performance is typical of small panels in hybridization capture enrichment.
The xGen Human ID Research Panel is designed to be used as a spike-in panel to larger capture panels. When used as recommended, the Human ID Research Panel does not affect target specificity and coverage uniformity of the original panel.
Figure 1. On-target performance of large and small recipient panels is unchanged by addition of xGen Human ID Research Panel. DNA libraries were prepared from NA12878 genomic DNA (Coriell) using a KAPA Hyper Prep Kit (Kapa Biosystems). Libraries were enriched with the xGen Exome Research Panel (>400,000 probes) or a custom panel comprised of 500 xGen Lockdown Probes, with and without spike in of the xGen Human ID Panel, using the recommended protocol. The enriched library was sequenced on a MiSeq® System (Illumina). The averages of on-target values (with 150 bp flank) across experiments are shown.