Processing
xGen™ Human Identification (ID) Hybridization Panel
Uniquely identify research samples by SNPs
The IDT xGen™ Human ID Hyb Panel enables unique identification of individual samples from a larger population. The panel provides genomic target enrichment of a distinctive set of single nucleotide polymorphisms (SNPs), including high minor allele frequency SNPs and a set of 5 gender marker SNPs. With a power of discrimination greater than 1 in 5 million, the xGen Human ID Hyb Panel is compatible with various sample types, including cell-free DNA and DNA from formalin-fixed, paraffin-embedded (FFPE) tissues.
xGen NGS—made for human gene identification research.
Ordering
- Trace NGS sample identity during data analysis
- Use as a spike-in or standalone panel with unique, modular format
- Consists of individually synthesized and quality-controlled 5’ biotin-modified hybridization capture probes
- Compatible with FFPE and cfDNA DNA samples
For the xGen Hybridization and Wash Kit, xGen Universal Blockers, xGen Library Amplification Primer Mix, and/or xGen Human Cot DNA, please visit the xGen Hybridization Capture Core Reagents page.
Product details
Capturing 76 distinct, highly polymorphic sites, the xGen Human ID Hyb Panel enriches a definitive set of single nucleotide polymorphisms (SNPs), selected to identify a unique individual from a population of over 5 million biological research samples. The panel has been tested with DNA from formalin-fixed, paraffin-embedded (FFPE) tissues, cell-free DNA (cfDNA), and other research sample types.
The xGen Human ID Hyb Panel comprises 229 individually synthesized hybridization capture probes, each quality controlled by ESI mass spectrometry. The xGen Human ID Hyb Panel can be combined with any xGen Custom Hyb Panel as a spike-in panel in research studies. When used as a spike-in to other panels, the data had similar on-target percentages. The xGen Human ID Hyb Panel can also be run as a standalone† panel to confirm sample identity before performing larger, more expensive sequencing studies.
Use the xGen Human ID Hyb Panel to:
- Identify tumor/normal paired research samples
- Track and manage individual research samples from one experiment to the next
- Identify research sample misidentification
†The xGen Human ID Hyb Panel contains a relatively small number of probes. When used as a standalone panel, expect lower on-target capture results than when used as a spike-in to a larger panel. This is typical of small panels in hybridization capture enrichment.
Product data
xGen Human ID Hyb Panel as a spike-in for sample identification
The xGen Human ID Hyb Panel can be used as a spike-in panel to larger capture panels in research studies. The xGen Human ID Hyb Panel does not affect target specificity and coverage uniformity of a representative xGen Custom Hyb Panel (Figure 1 and Figure 2).
Figure 1. Example showing the spike in of the xGen Human ID Hyb Panel does not impact on-target performance of recipient panel. DNA libraries were prepared from NA12878 genomic DNA. 500 ng of the libraries were enriched with a custom panel comprised of 509 xGen Hyb Probes, with (n=8) and without spike in (n=3) of the xGen Human ID Research Panel. The enriched libraries were sequenced on a MiSeq® System (Illumina) using 2 X 150 paired-end reads. Each sample was sub-sampled to 0.5M total reads. The average of on-target rate (with 150 bp flank) across experiments are shown.
Figure 2. Example of uniform coverage of targeted regions using xGen Human ID Hyb Panel. Genomic DNA libraries were enriched using the xGen Human ID Hyb Panel spiked into a custom panel containing 509 probes (n=8). Enriched samples were sequenced on a MiSeq system using 2 x 150 paired-end reads. Total reads for each sample were sub-sampled to 0.5M total reads. The average normalized target coverage ranged between 0.60X and 1.51X for the regions targeted by the human ID Panel. Normalized target coverage bounds at 0.5X and 1.5X are denoted by the doted lines.
Resources
RUO21-0304_001