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xGen™ Oncology & Inherited Diseases Amplicon Panels

Compatible with FFPE and cell-free DNA samples

Confident variant identification as low as 1% allele frequency facilitates research on tumor growth and identification of driver mutations in a fast, easy, single-tube workflow.

xGen NGS—made for oncology and inherited disease research.

Ordering

xGen Amplicon PanelSpecifications linkBED Files link/download
Oncology Research PanelsxGen 56G Oncology Amplicon PanelSpecificationsPanel Targets
xGen 57G Pan-Cancer Amplicon PanelSpecificationsPanel Targets
xGen TP53 Amplicon PanelSpecificationsPanel Targets
xGen EGFR Pathway Amplicon PanelSpecificationsPanel Targets
xGen BRCA1/BRCA2 Amplicon PanelSpecificationsPanel Targets
xGen BRCA1/BRCA2 PALB2 Amplicon PanelSpecificationsPanel Targets
xGen Colorectal Amplicon PanelSpecificationsPanel Targets
xGen Lung Amplicon PanelSpecificationsPanel Targets
xGen Lynch Syndrome Amplicon PanelSpecificationsPanel Targets
xGen Myeloid Amplicon PanelSpecificationsPanel Targets
Inherited Disease Research PanelxGen CFTR Amplicon PanelSpecificationsPanel Targets
  • Compatible with Illumina® sequencing platforms
  • Designed for research on germline and somatic variant identification, or targeted sequencing
  • Offers overlapping amplicons in a fast, easy, single-tube workflow

Figure 1. Plate combinations for 1536 UDI primer pairs. Each 96-well plate is sold in a set of four plates. For 1536 UDI primers, order Set 1, 2, 3, and 4.

For plate layouts of each of the 16 UDI plates offered see the xGen™ Normalase™ Indexing Primers quick reference guide.

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Want to learn more about our xGen™ Amplicon Panels—developed with super amplicon technology—and how to customize your panel or spike-in genes in our predesigned panels? Your time is valuable—we’ll prioritize your inquiry and be in touch to discuss it ASAP.

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Product details

xGen Amplicon Panels for oncology and inherited diseases research utilize multiple overlapping amplicons in a single tube, using a rapid, 2.5-hour workflow to prepare ready-to-sequence libraries in research studies (Figure 2). The PCR1+PCR2 workflow generates NGS libraries for identifying genetic changes in the genes associated with a variety of tissues and inherited diseases (Table 1). The libraries may be quantified with conventional methods such as Qubit® (Thermo Fisher Scientific) or Agilent Bioanalyzer® and normalized by manual pooling or normalized enzymatically with the included xGen Normalase™ reagents.

Figure 2. xGen Amplicon Panels have a single tube workflow that is done in as little as 2.5 hours. Creating an NGS library starts with multiplex PCR. Your custom or predesigned panel is combined with the DNA sample to amplify the targets of interest. The samples are then amplified with indexing primers to create a functional dual indexed library. As an optional step, the xGen Normalase reagent can be used after pooling multiple libraries to ensure each is equally represented in the final sample for the flowcell. 

Table 1. List of xGen Oncology & Inherited Diseases Amplicon Panels

PanelNumber of GenesGenes
xGen 56G Oncology Amplicon Panel v2, 96 rxn56ABL1, AKT1, ALK, APC, ATM, BRAF, CDH1, CDKN2A, CSF1R, CTNNB1, DDR2, DNMT3A, EGFR, ERBB2, ERBB4, EZH2, FBXW7, FGFR1, FGFR2, FGFR3, FLT3, FOXL2, GNA11, GNAQ, GNAS, HNF1A, HRAS, IDH1, IDH2, JAK2, JAK3, KDR, KIT, KRAS, MAP2K1, MET, MLH1, MPL, MSH6, NOTCH1, NPM1, NRAS, PDGFRA, PIK3CA, PTEN, PTPN11, RB1, RET, SMAD4, SMARCB1, SMO, SRC, STK11, TP53, TSC1, VHL
xGen 57G Pan-Cancer Amplicon Panel, 96 rxn57ABL1, AKT1, ALK, APC, ATM, BRAF, CDH1, CDKN2A, CSF1R, CTNNB1, DDR2, DNMT3A, EGFR, ERBB2, ERBB4, EZH2, FBXW7, FGFR1, FGFR2, FGFR3, FLT3, FOXL2, GNA11, GNAQ, GNAS, HNF1A, HRAS, IDH1, IDH2, JAK2, JAK3, KDR, KIT, KRAS, MAP2K1, MET, MLH1, MPL, MSH6, NOTCH1, NPM1, NRAS, PDGFRA, PIK3CA, PTEN, PTPN11, RB1, RET, SMAD4, SMARCB1, SMO, SRC, STK11, TP53, TSC1, TSC2, VHL
xGen BRCA1 BRCA2 Amplicon Panel, 96 rxn2BRCA1, BRCA2
xGen BRCA1 BRCA2 PALB2 Amplicon Panel, 96 rxn3BRCA1, BRCA2, PALB2
xGen CFTR Amplicon Panel, 96 rxn1CFTR (All exons including 5’ and 3’ UTRs, select intronic regions (1, 12, 22, and 25))
xGen EGFR Pathway Amplicon Panel, 96 rxn4EGFR, BRAF, KRAS, NRAS
xGen Colorectal Amplicon Panel, 96 rxn16AKT1, APC, BRAF, ERBB2, ERBB4, KIT, KRAS, NOTCH1, NRAS, PDGFRA, PIK3CA, POLE, PTEN, SMAD4, STK11, TP53
xGen TP53 Amplicon Panel, 96 rxn1TP53
xGen Lung Amplicon Panel, 96 rxn17AKT1, ALK, ARAF, BRAF, EGFR, ERBB2, ERBB4, FGFR1, FGFR2, FGFR3, KRAS, MAP2K1, MET, NRAS, PIK3CA, PTEN, TP53
xGen Lynch Syndrome Amplicon Panel, 96 rxn4MLH1, MSH2, MSH6, PMS2
xGen Myeloid Amplicon Panel, 96 rxn23ASXL1, CALR, CEBPA, CSF3R, DNMT3A, EZH2, FLT3, HRAS, IDH1, IDH2, JAK2, JAK3, KDM6A, KIT, MPL, NPM1, RUNX1, SETBP1, SF3B1, SRSF2, TET2, TP53, U2AF1

Bold indicates whole CDS coverage

RUO21-0279_003.1