IDT is committed to providing quality products to researchers working on the cutting edge of scientific discovery. The xGen Monkeypox Virus Amplicon Panel was designed as part of the next generation sequencing (NGS) Tech Access program at IDT, which is
intended to accelerate innovation by enabling earlier access to our most advanced research tools. Tech Access products have not been through our standard, rigorous development cycle. These products are particularly well suited for researchers who
require the most up-to-date technology to unlock new discoveries.
The current variants of monkeypox that are circulating have a genome of nearly 200 kb double-stranded DNA . Surveillance of the virus and any potential mutations have gained international support due to the lessons learned through the COVID-19 pandemic.
The IDT xGen Monkeypox Virus Amplicon Panel helps to enable researchers to track monkeypox strains, including potential new variants, by next generation sequencing.
DNA-to-sequencer in 2.5 hours
The workflow for the xGen Monkeypox Virus Amplicon Panel starts with extracted viral DNA (Figure 1). You can then generate an NGS library in a single tube using tiled primer pairs designed to target 184 kb of the monkeypox genome. Primers were designed
for the currently circulating strain of the monkeypox virus (NCBI accession number ON568298 ), which allows you to generate overlapping amplicons in a single-tube, PCR 1 + PCR 2 workflow. If pooling multiple samples for NGS, the xGen Amplicon Core
Kit includes the reagents for Normalase™ technology, the proprietary enzymatic normalization step that reduces hands-on time needed for manual normalization. Specifications of the Tech Access, xGen Monkeypox Virus Amplicon Panel are found in