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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Do custom oligos come with 5’ phosphorylation?

Synthetic DNA and RNA are synthesized with 5' and 3' OH groups. If your application requires a 5' phosphate group, such as a ligation reaction, include a phosphorylation modification as part of your sequence. The modification code for 5' phosphorylation is /5Phos/ and would be inserted into your sequence at the 5' end.

Custom DNA & RNA

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.