The minimum coverage per assay will depend on the target variant allele frequency (VAF) of the experiment. Lower VAF values will require more reads to see rare variants.
Assuming a 50% VAF, we recommend calling SNPs at targets covered by a minimum of 20 reads.
*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.