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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How can I ensure that I am designing my CRISPR-Cas9 crRNA protospacer sequence in the correct orientation and to the appropriate strand?

The CRISPR-Cas9 crRNA recognizes and binds 19–20 nt on the genomic DNA strand opposite to the NGG sequence of the PAM site. We recommend using 20 nt upstream of the PAM site in the forward orientation as your crRNA protospacer sequence.

Also, make sure you verify the correct strand orientation of the protospacer sequence and do not include the PAM site.