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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How do I calculate the melting temperature (Tm) for oligos containing locked nucleic acids such as Affinity Plus bases?

Use the free, online IDT OligoAnalyzer Tool to calculate the melting temperature (Tm) for oligos containing locked nucleic acids such as Affinity Plus modifications.

To include an Affinity Plus base in your sequence, simply place “+” in front of the base, e.g., +A+C+G+T.

Learn more about Affinity Plus DNA & RNA Oligonucleotides.




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*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.