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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Is a PAM sequence present in every gene?

This is hard to say if pam sequence is in every gene. Because the frequency of the dinucleotide ‘GG’ is 5.21% in the human genome [1], it is reasonable to expect that most genes will contain the NGG sequence of the S. pyogenes PAM.

References:

  1. Scherer S Cold Spring Harbor Laboratory. Press., A short guide to the human genome2008, Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press. xiv, 173 p.


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