Library amplification may not be required, depending on the amount of library needed for your downstream application. However, if library amplification is not performed (i.e., when doing a PCR-free workflow with ≥100 ng of library input), full-length adapters must be used for adapter ligation.
*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.