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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Is the PrimeTime Gene Expression Master Mix appropriate for qPCR assays that use intercalating dyes (e.g., SYBR® Green and EvaGreen® dyes)?

No. PrimeTime™ Gene Expression Master Mix is compatible with probe-based assays (such as all PrimeTime qPCR Assays from IDT and TaqMan® Assays from Thermo Fisher Scientific). 

SYBR is a registered trademark of Life Technologies, Inc.
EvaGreen dye is a registered trademark of Biotium.


*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.