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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Is the PrimeTime® Gene Expression Master Mix compatible with different real-time PCR instruments or systems?

Yes. The master mix has been successfully used in qPCR experiments run on the QuantStudio™ 7 Flex (Thermo Fisher Scientific), 7900HT (Thermo Fisher Scientific), 7500 (Thermo Fisher Scientific), the CFX384™ (BioRad), and the LightCycler® 480 (Roche) instruments. All such experiments used the cycling conditions provided in the protocol, 
PrimeTime Gene Expression Master Mix.

Reference dye is provided in a separate tube, so you may add it to the master mix at the levels required by your real-time PCR instrument.


*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.