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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

What is the concentration of the Alt-R™ Cas9 nucleases and nickases?

The Alt-R Cas9 nucleases and nickases are supplied at concentration of 10 mg/mL in 25 mM Tris-Cl, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol (v/v), pH 7.4, with the exception of the Alt-R Cas9, glycerol-free. The Alt-R Cas9, glycerol-free is provided in at a concentration of 10 µg/mL in a buffer without glycerol.

These proteins are provided with a purity guarantee of ≥95% pure and are tested to be free of detectable DNases/RNases and bacterial DNA, and contain less than 2 Endotoxin Units (EU)/mL endotoxin.

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.