A small aliquot (1 µL) can be taken from the fragmentation product for analysis using electrophoresis-based methods [e.g., Bioanalyzer instrument (Agilent), TapeStation® system (Agilent)]. You can increase the reaction volume to 31 µL to compensate for this QC step. We have analyzed samples on an HS Bioanalyzer chip without cleanup (Figure). Migration is not affected but signal is reduced in samples run directly, without cleanup, when compared to samples with 1.8X SPRI cleanup.
Figure. Electrophoretic analysis of fragmentation samples with and without cleanup shows similar migration but lower signal without cleanup. Lotus DNA Library Prep libraries (150 ng input NA12878, Coriell; diluted to 5 ng/μL) with and without 1.8X SPRI cleanup were analyzed on a Bioanalyzer HS chip.
*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.