Long, high-quality oligos for demanding applications such as cloning, ddRNAi, homology-directed repair, and gene construction
Ultramer DNA Oligonucleotides are generated by proprietary synthesis methods that deliver high-quality oligos up to 200 bases. They are available single- or double-stranded and can be delivered in tubes or plates.
NEW! Try our Alt-R™ HDR Donor Oligos; single-stranded DNA oligos specifically built for your homology-directed repair (HDR) experiments. Designed and optimized through extensive wet-bench testing, Alt-R HDR Donor Oligos are ideal for introducing point mutations and short insertions.
Formulated to 4 nmol or 20 nmol guaranteed yield. Shipped dry or resuspended to 100 µM in IDTE buffer, pH 8.0.
2 oligos, annealed and delivered in a single tube. Formulated to 4 nmol or 20 nmol guaranteed yield. Shipped dry.
The following annealing fees will be applied to each duplex ordered:
Shipped dry or resuspended to your specifications. Minimum 24 and 96 oligos required for 96- and 384-well plates, respectively. 200 pmol scale requires a minimum of 96 oligos.
1 oligo pair per well, annealed. Shipped dry or resuspended in duplex buffer. Minimum of 24 and 96 oligos required for 96- and 384-well plates, respectively.
For ordering inquiries, please contact us.
Ultramer DNA Oligos are long, single- and double-stranded synthetic DNA sequences. They are manufactured with an synthesis platform that consistently results in coupling efficiencies over 99.5%. Ultramer DNA Oligos can be used in a variety of molecular biology applications, including (but not limited to):
Every Ultramer DNA Oligo you receive will be deprotected and desalted to remove small molecule impurities. In addition, your oligos will be checked via electrospray ionization mass spectrometry (ESI-MS)* and quantitated by UV spectrophotometry.
*With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be evaluated by ESI‑MS.
Description | 200 pmol Ultramer DNA Oligo | 4 nmol Ultramer DNA Oligo | 20 nmol Ultramer DNA Oligo | PAGE Ultramer DNA Oligo |
---|---|---|---|---|
Availability | Plates | Plates and tubes | Plates and tubes | Tubes |
5' Phosphorylation | • | • | • | • |
5' Biotin | • | • | • | • |
5' Amino Modifier C6 | • | • | • | |
5' Amino Modifier C12 | • | • | • | |
5' deoxyInosine | • | • | • | • |
5' deoxyUridine | • | • | • | • |
Int deoxyInosine | • | • | • | • |
Int deoxyUridine | • | • | • | • |
Phosphorothioate Bond | • | • | • | • |
2' O-Methyl RNA bases | • | • | • | • |
3' Amino Modifier | • | • | ||
5' 5-Methyl dC | • | • | • | |
Int 5-Methyl dC | • | • | • | |
Int Spacer 18 | • | • | • | |
3' C3 Spacer | • | • | • |
IDT proprietary DNA synthesis equipment permits rapid, high-quality synthesis of nucleic acids. This platform is adjusted to a lower throughput mode which uses an "extra rich" synthesis cycle for long oligos. Along with this refined synthesis cycle, Ultramer DNA Oligos use a solid support that is specifically designed to synthesize low-yield, high-quality oligos up to 200 bases in length. Overall, these improvements to our already established manufacturing methods allow you to acquire longer, purer oligos for your research. Figure 1 illustrates the quality of Ultramer DNA Oligos.
Figure 1. IDT proprietary platforms have a better coupling efficiency than other suppliers, which provides more full-length oligonucleotides in your order. Small increases in coupling efficiency (≤1%) result in measurable increases in full-length product yield. The curves represent the theoretical yield for different lengths of oligos based on a coupling efficiency of 99.4% (IDT Oligos, n = 126) and 99.1% (other suppliers, n = 134 from three different suppliers) using the formula, percent full length product = (eff )(n-1)*100 where eff = coupling efficiency (for example, 99.4% = 0.994) and (n–1) is the number of coupling reactions needed to make an oligo of length n.
Our proprietary ESl-MS methods allow us to confidently provide quality control documentation for your oligos up to 200 bases in length.* These QC traces, such as the examples shown in Figure 2, are available online free of charge.
*With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be evaluated by ESI-MS.
Diverse laboratory configurations and various individual preferences in the field require oligos that can be delivered in unique formats. With the formulation services listed below, you can have your oligos tailor-made to your specific requirements. Learn more »
In addition to the free ESI-MS analysis that comes standard with your oligos,* your project may require one of the analytical services listed below. Learn more »
*With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be evaluated by ESI‑MS.
If you require oligos that are ISO 13485:2016 certified, or if you are interested in our third-party manufacturing services, please click here.
DNA with homology to the sequences flanking a double-stranded break (DSB) can serve as template for error-free homology directed-repair (HDR) of the DSB. The efficiency of HDR is determined by the concentration of donor DNA present at the time of repair, length of the homology arms, cell cycle, and activity of the endogenous repair systems in the particular cell [1]. These factors contribute to the high variability of HDR efficiency observed across different cell lines, and particularly in immortalized cells [2]. Typically, in replicating mammalian cells, donor arms are at least 500 bp in length [3]. However, it is important to determine the optimal HDR conditions for your cell line.
Inserts between the homology arms are frequently in the 1–2 kb range [4]. While longer inserts are possible, the efficiency of recombination decreases as the insert size increases [5]. Finding successfully integrated inserts is likely to be challenging when inserts are greater than 3 kb in most mammalian cells.
Single-stranded oligo DNA (ssODN) has recently been identified as a substrate that is preferred by the HDR mechanism and often achieves good efficiency with homology arms as short as 40 bp [6,7]. The drawback to using ssODNs is that they are limited in length to a few hundred bases, so the insert size is limited. When using Ultramer® oligos as templates for a short insertion, tag, or SNP conversion, we have found arm lengths of 30–60 nt to be sufficient.
References
GMP refers to products manufactured under ISO 13485: 2016 QMS. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations for their legal marketing.
*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.