Integrated DNA Technologies acquires Archer™ next generation sequencing research assays to advance actionable scientific discoveries.

Order by stock part number »
Get Help
 |  EN
Sign In
ProcessingProcessing
  1. Products
  2. DNA & RNA
  3. Oligo modifications
22_DR_ProductOverview_HeaderIcon_DNARNA_180x180

Oligo modifications

IDT offers a vast range of oligo customization options, including fluorophores, dark quenchers, modified bases, linkers, spacers, click chemistry, and more.

Attachment chemistry and linkers

Link an oligonucleotide with another molecule or a particular surface. Subcategories include biotins, amino­ modifiers, alkynes, and thiol modifiers, as well as azide, digoxigenin, and cholesterol-TEG.

Learn more

Fluorophores and dark quenchers

Fluorescent dyes re-emit light upon excitation while dark quenchers absorb the emitted light and release heat. These modifications are added to oligos that are used in a variety of molecular biology applications. Browse our Freedom™ Dyes, which have no patent licensing restrictions, or the full catalog of fluorophores and dark quenchers.

Learn more

Modified bases

A variety of modifications that can serve a range of functions, including cross-linking, duplex stabilization, and nuclease resistance.

Learn more

Phosphorylation

Use if your oligo is being used as a substrate for DNA ligase. 3' phosphorylation will inhibit degradation by some 3' exonucleases and can be used to block extension by DNA polymerase.

Learn more

Spacers

Create distance between a functional moiety and the hybridizing region of your oligo. This category includes the commonly used C3 Spacer, as well as Spacer 9, Spacer 18, and dSpacer.

Learn more

Click chemistry modifications

Learn about the two-step process that uses quantitative chemical reactions of alkyne and azide moieties to create covalent carbon-heteroatom bonds between biochemical species. See available modifications.

Learn more

Phosphorothioate bonds

Include these bonds in your oligo sequence for increased inter-nucleotide resistance to nuclease degradation. These modified bonds are especially beneficial when incorporated into antisense oligos.

Learn more

Overview

A wide variety of modifications can be incorporated into an oligo at the time of synthesis. Some modifications, however, must be attached after synthesis, using NHS Ester chemistry. These post-synthesis modifications often result in lower yields, as they require HPLC purification.

For detailed information about the modifications we offer, click on any of the categories above. Note that not all modifications are available for all types of products and sequences. If the modification you need is not listed, please contact us.

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.

RUO22-1371_001